Topology of sarcoplasmic reticulum Ca2+-ATPase: An infrared study of thermal denaturation and limited proteolysis
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چکیده
منابع مشابه
Ca2+-ATPase activity and Ca2+ uptake by sarcoplasmic reticulum in fish heart: effects of thermal acclimation.
This study was designed to compare the activities of sarcoplasmic (SR) Ca2+-ATPase and Ca2+ uptake in fish and mammalian hearts and to determine whether thermal acclimation has any effect on the function of the cardiac SR in fish. To this end, we measured thapsigargin-sensitive Ca2+-ATPase activity and thapsigargin-inhibitable Ca2+ uptake velocity in crude cardiac homogenates of newborn and adu...
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Vanadate induces the formation of two-dimensional crystalline arrays of Ca2+-ATPase molecules in sarcoplasmic reticulum. The Ca2+-ATPase membrane crystals are evenly distributed among the terminal cisternae and longitudinal tubules of sarcoplasmic reticulum, but very few crystals were observed in the T tubules. Tryptic cleavage of the Ca2+ transport ATPase into two major fragments (A and B) did...
متن کاملThe structure of the Ca2+-ATPase of sarcoplasmic reticulum.
In this article the morphology of sarcoplasmic reticulum, classification of Ca(2+)-ATPase (SERCA) isoenzymes presented in this membrane system, as well as their topology will be reviewed. The focus is on the structure and interactions of Ca(2+)-ATPase determined by electron and X-ray crystallography, lamellar X-ray and neutron diffraction analysis of the profile structure of Ca(2+)-ATPase in sa...
متن کاملOn subunit structure of Ca2+-dependent ATPase of sarcoplasmic reticulum.
The protein composition of sarcoplasmic reticulum membranes has been extensively characterized by means of polyacrylamide gel electrophoresis in one [l] or two [2] dimensions. It is generally accepted that the protein of mol. wt 100 000 (i.e., Ca’+ATPase) is the principal protein of sarcoplasmic reticulum. However, monomer and dimer fractions of deoxycholate-solubilized Ca”-ATPase during isoele...
متن کاملVariation of scallop sarcoplasmic reticulum Ca2+-ATPase activity with temperature.
Methods for preparing native scallop sarcoplasmic reticulum vesicles, largely purified membranous scallop sarcoplasmic reticulum Ca2+-ATPase, and nonionic detergent-solubilized sarcoplasmic reticulum Ca2+-ATPase are described. The effect of a range of polyoxyethylene-based detergents on the solubilized Ca2+-ATPase was tested. Decaethylene glycol dodecyl ether (C12E10) supported the highest leve...
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ژورنال
عنوان ژورنال: Protein Science
سال: 1998
ISSN: 0961-8368,1469-896X
DOI: 10.1002/pro.5560070511